BD Biosciences FACSCount

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FACSCount

The BD FACSCount system is a proven, turnkey instrument and reagent system designed for CD4 counting to stage HIV/AIDS, guide treatment decisions for HIV-infected persons, and to evaluate effectiveness of therapy in a wide range of laboratory settings. As the system of choice for CD4 monitoring in global HIV/AIDS treatment programs for over a decade, the BD FACSCount system is recognized for its simplicity of operation, robust performance, and reliable results. Today clinicians in programs throughout Africa, Asia, Eastern Europe, and Latin America continue to rely on the system for cost-effective, simple, and dependable CD4 testing.
FACSCount
Attributes
Additional Specifications System Performance
Sample stability 48 hours after preparation when stored at room temperature (20° to 25°C, 68° to 77°F)

Whole blood stability
  • BD FACSCount Reagents and BD FACSCount CD4/CD3 Reagents 48 hours after draw when stored at room temperature (20° to 25°C, 68° to 77°F)
  • BD FACSCount CD4 Reagents 24 hours after draw when stored at room temperature (20° to 25°C, 68° to 77°F)

    User reportable range
  • The following ranges meet BD performance characteristics.
  • BD FACSCount Reagents and BD FACSCount CD4/CD3 Reagents*
  • CD4+: 50 to 2,000 cells/µL
  • CD8+: 100 to 2,000 cells/µL
  • CD3+: 100 to 3,500 cells/µL

    BD FACSCount CD4 Reagents
    CD4+: 50 to 5,000 cells/µL 5% to 65%

    Precision
    <10% CV

    BD FACSCount Instrument

    Laser
    Convection-cooled laser

    Sample flow rate
    60 µL/min ±10%

    Power
  • 120 VAC ±10% 60 Hz ±5%
  • 100 VAC ±10% 60 Hz ±5%
  • 220/240 VAC ±10% 50 Hz ±5%
  • 160 W (maximum rated power)

    Dimensions
  • Width: 43.2 cm (17 in.)
  • Height: 38.1 cm (15 in.)
  • Depth: 55.9 cm (22 in.)

    Weight
    25.9 kg (57.1 lb), fluid reservoirs empty

    Heat dissipation
    <146.5 (<500 BTU/h)

    Fluid reservoir capacity
    2 L

    Fuses
    2.5 A (T), 250 VAC (IEC127), SEMKO approved 5 x 20-mm fuse

    Regulatory status
    For In Vitro Diagnostic Use in the United States

    Instrument Accessories

    Barcode reader
    The optional barcode reader reads the following barcode standards:
    • Code 39
    • Code-a-bar
    • Interleaved 2 of 5
    • Universal Product Code (UPC)
    • European Article Numbering (EAN)

    Workstation
    Provides a place to hold supplies while preparing and running samples

    Dimensions:
  • Width: 48.3 cm (19 in.)
  • Height: 9.1 cm (3.6 in.)
  • Depth: 18.5 cm (7.3 in.)

    Electronic pipette
    Preprogrammed to accurately deliver 50 µL of fluid

    Coring station
    Used to open reagent and control tubes
  • Features

    Easy to Install, Set Up, and Run with Confidence
    The BD FACSCount system is easy to install. The instrument operates from a standard electrical outlet, requiring no hardware adjustments or calibration. Only minimal training is needed to set up the instrument and to run samples. Simple prompts on the display guide operators through testing, from input of patient information to final results. The software automatically identifies the lymphocyte populations of interest and summarizes patients’ results in a printed sample report. Quality controls in the software ensure that reported results are accurate by detecting and flagging error conditions and suppressing results when control limits are exceeded. Results are objective, requiring no interpretation or subjective analysis by the operators.

    Quality control
    To validate laboratory practice and methodology, BD FACSCount™ control beads can be added to samples prepared with normal blood. BD FACSCount fluorescent reference beads are included in the reagent tube, and ensure accurate enumeration of lymphocytes of interest with no operator intervention. The control run generates a detailed analysis in a printed report summarizing system performance. The result of the last control run is reported on subsequent sample printouts to help determine linearity and pipetting accuracy. Since absolute counts are calculated from directly measured cell counts and reference bead counts on the BD FACSCount system, no additional analysis on a hematology analyzer is necessary.
    Simplified sample handling
    Sample preparation follows an easy three-step process. First, blood is added to a tube that contains pre-measured reagents. Then the sample is vortexed and incubated. In the second step, a fixative is added to the tube, and then it is vortexed and incubated. In step three, the sample is vortexed and run on the instrument.
    Easy three-step sample preparation
    Prepare controls and patient samples by adding blood, then fixative solution, to the reagent tubes.

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    Manuals and Resources

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    Instrument Documents

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    Questions and Answers

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    annual maintenance of bd facs count

    Please what is the required volume of sample which needs to have been assayed on the BD FACS COUNT  before an annual maintenance can be done?

    Dépannage

    Bonjour Quand j'éteint mon facscount il faut à chaque démarrage réglé l'heure pourquoi? Merci  

    DATA SHEET OF SECIRITE OF REAGENT BD FACSCount / FICHE DE DONNEE SECIRITE DES REACTIF DU BD FACSCOUNT

    Translation of originial post: Hello, I am responsible for security in my laboratory at the General Hospital Issia in Ivory Coast where a nousutilison FASCount. I wish I had the safety data sheets on the reagents used in this equipment...

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