The BD FACSCount system is a proven, turnkey instrument and reagent system designed for CD4 counting to stage HIV/AIDS, guide treatment decisions for HIV-infected persons, and to evaluate effectiveness of therapy in a wide range of laboratory settings. As the system of choice for CD4 monitoring in global HIV/AIDS treatment programs for over a decade, the BD FACSCount system is recognized for its simplicity of operation, robust performance, and reliable results. Today clinicians in programs throughout Africa, Asia, Eastern Europe, and Latin America continue to rely on the system for cost-effective, simple, and dependable CD4 testing.
||System Performance |
Sample stability 48 hours after preparation when stored at room temperature (20° to 25°C, 68° to 77°F)
Whole blood stability BD FACSCount Reagents and BD FACSCount CD4/CD3 Reagents 48 hours after draw when stored at room temperature (20° to 25°C, 68° to 77°F) BD FACSCount CD4 Reagents 24 hours after draw when stored at room temperature (20° to 25°C, 68° to 77°F)
User reportable range The following ranges meet BD performance characteristics. BD FACSCount Reagents and BD FACSCount CD4/CD3 Reagents* CD4+: 50 to 2,000 cells/µL CD8+: 100 to 2,000 cells/µL CD3+: 100 to 3,500 cells/µL
BD FACSCount CD4 Reagents
CD4+: 50 to 5,000 cells/µL 5% to 65%
BD FACSCount Instrument
Sample flow rate
60 µL/min ±10%
Power 120 VAC ±10% 60 Hz ±5% 100 VAC ±10% 60 Hz ±5% 220/240 VAC ±10% 50 Hz ±5% 160 W (maximum rated power)
Dimensions Width: 43.2 cm (17 in.) Height: 38.1 cm (15 in.) Depth: 55.9 cm (22 in.)
25.9 kg (57.1 lb), fluid reservoirs empty
<146.5 (<500 BTU/h)
Fluid reservoir capacity
2.5 A (T), 250 VAC (IEC127), SEMKO approved 5 x 20-mm fuse
For In Vitro Diagnostic Use in the United States
The optional barcode reader reads the following barcode standards:
• Code 39
• Interleaved 2 of 5
• Universal Product Code (UPC)
• European Article Numbering (EAN)
Provides a place to hold supplies while preparing and running samples
Dimensions: Width: 48.3 cm (19 in.) Height: 9.1 cm (3.6 in.) Depth: 18.5 cm (7.3 in.)
Preprogrammed to accurately deliver 50 µL of fluid
Used to open reagent and control tubes
Easy to Install, Set Up, and Run with Confidence
The BD FACSCount system is easy to install. The instrument operates from a standard electrical outlet, requiring no hardware adjustments or calibration. Only minimal training is needed to set up the instrument and to run samples. Simple prompts on the display guide operators through testing, from input of patient information to final results. The software automatically identifies the lymphocyte populations of interest and summarizes patients’ results in a printed sample report. Quality controls in the software ensure that reported results are accurate by detecting and flagging error conditions and suppressing results when control limits are exceeded. Results are objective, requiring no interpretation or subjective analysis by the operators.
To validate laboratory practice and methodology, BD FACSCount™ control beads can be added to samples prepared with normal blood. BD FACSCount fluorescent reference beads are included in the reagent tube, and ensure accurate enumeration of lymphocytes of interest with no operator intervention. The control run generates a detailed analysis in a printed report summarizing system performance. The result of the last control run is reported on subsequent sample printouts to help determine linearity and pipetting accuracy. Since absolute counts are calculated from directly measured cell counts and reference bead counts on the BD FACSCount system, no additional analysis on a hematology analyzer is necessary.
Simplified sample handling
Sample preparation follows an easy three-step process. First, blood is added to a tube that contains pre-measured reagents. Then the sample is vortexed and incubated. In the second step, a fixative is added to the tube, and then it is vortexed and incubated. In step three, the sample is vortexed and run on the instrument.
Easy three-step sample preparation
Prepare controls and patient samples by adding blood, then fixative solution, to the reagent tubes.
Manuals and Resources
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