Thermo Fisher Scientific - Thermo Fisher 7900HT PCR
by Thermo Fisher Scientific

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DESCRIPTION

Real-Time PCR System

The 7900HT Fast Real-Time PCR System is a real-time quantitative PCR system that combines 96- and 384-well plate compatibility and the TaqMan Low Density Array with fully automated robotic loading-and now also offers optional Fast real-time PCR capability.

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-Engr Michael Okeke
a year ago
Instrument reports all samples as empty wells
I have an ABI 7900HT that reports empty wells for all samples. The intensity is all below recommended amplitude 10,000. I need a guide on how to adjust the intensity of the signals.Reply
DOCUMENTS / MANUALSView All

FEATURES

  • Fast PCR option reduces run time to about 35 minutes in a standard 96-well format, or about 55 minutes in a 384-well plate
  • Continuous wavelength detection from 500-660 nm allows the use of multiple fluorophores in a single reaction
  • Measure gene expression levels, detect and quantitate pathogens, perform allelic discrimination (SNP genotyping) assays as well as score the presence of gene sequences
  • 96- or 384-well plate compatibility (including the TaqMan Low Density Array)
  • Optional Enterprise edition software provides data analysis tools that support 21 CFR Part 11 guidelines providing data integrity and security
  • Hands-free plate-loading and unloading provides true walkaway automation allowing users to increase user lab's productivity
  • Proven assay development guidelines save time and money

Additional Specifications

  • High Throughput
    The 7900HT System is a high-throughput real-time PCR system that detects and quantitates nucleic acid sequences. An optional Automation Accessory (not included) combined with 384-well plate capability make the 7900HT system ideally suited to meet the high-throughput requirements of today's drug discovery process. Key applications include gene expression quantitation and the detection of single nucleotide polymorphisms (SNPs) using the fluorogenic 5' nuclease assay.
  • Flexibility
    The 7900HT system is a versatile research tool that can accommodate any real-time PCR need. User-interchangeable thermal cycling block formats lets users select the format that’s right for projects, using industry-standard 96- and 384-well formats, as well as a novel 384-well TaqMan Low Density Array and a new Fast 96-well block that reduces run times from 2 hours to about 30 minutes. An easy automation upgrade path lets users add features to meet throughput demands. A Fast PCR option reduces run times from 2 hours to about 30 minutes.
  • Powerful Software
    Using two automation software tools, Plate Utility and Automation Controller, most of the gene expression and SNP genotyping workflow is fully automated for high-throughput applications and requires minimal user intervention. Users can now process 5,000 x 384 well plates (384 markers), which equates to 1.92 million genotypes. Using Relative Quantification (RQ) Manager, users can also process 200 x 384-well plates (96 detectors with quadruplicate data points⁄multiplexed endogenous control), which equates to 153,600 data points. Fully integrated into one complete Enterprise system, SNP Manager and RQ Manager eliminate data analysis bottlenecks in high-throughput SNP and gene expression research by allowing significantly more plates to be analyzed simultaneously. This reduces hands-on analysis time and simplifies the data analysis workflow.

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