Agilent Technologies - Seahorse XF HS Mini
by Agilent Technologies

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DESCRIPTION

XF Analyzer

Agilent Seahorse XF HS Mini analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in an eight-well miniplate format. These measurements are critical elements of energy metabolism and vital indicators of mitochondrial health, toxicity, and overall cellular (dys)function. Together, these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples.


The XF HS Mini analyzer is easy to set up and operate and has excellent OCR/PER precision. It is also compatible with the XF HS miniplate, which can generate robust assay signals using three times fewer cells compared to the XFp miniplate—making it good for studies when biological materials are limited.

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FEATURES

  • Live cell, real-time analysis of cellular energy metabolism platform in eight-well format
  • Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP product rates
  • Excellent OCR precision at low rates, providing confidence when working with quiescent cell types, such as naïve T cells
  • High sensitivity that requires 3x fewer cells to achieve robust response, most suited for studies with limited biological materials when working together with XF HS miniplates
  • Four-port injection system with automated mixing feature for assessing immediate cellular responses to substrates, inhibitors, and other compounds in real time
  • Wide operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
  • Web-based analytics tool, Seahorse Analytics, for streamlined data analysis

Additional Specifications

Controller Description    

  • Integrated computer/monitor Windows 10 (64 bit) OS and touch screen interface for full instrument control, basic assay design, and data viewing

Dimensions (WxDxH)    

  • 30 cm x 58 cm x 43 cm

Operating Environment Relative Humidity    

  • 20-70 %

Operating Environment Temperature    

  • 4 - 30 °C

Power Requirements    

  • 100-240 VAC, 6 A, 50/60 Hz

Sample Requirements    

  • 1500 - 250000 cells/well

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