Accuri Cytometers - C6 Flow Cytometer
by Accuri Cytometers

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DESCRIPTION

Flow Cytometer 

BD Accuri C6 flow cytometers, BD CFlow® Software and Workstation PC supply all the capabilities of a full-featured flow cytometer at an affordable price. The system includes blue and red lasers, four color detectors, and both forward and side scatter detectors plus software that is so intuitive that you will typically be up and running within an hour of receiving your BD Accuri system.

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-Joel Gaikwad
6 years ago
sheath fluid

My BD Accuri C6 displays the message- refill sheath fluid- while the tank is almost full?

Takes more than usual time to analyze samples?

any hints? what is the problem?

thanks Joel

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DOCUMENTS / MANUALSView All

FEATURES

  • Decade dynamic range: The totally digital C6 Flow Cytometer has state-of-the-art electronics that make it possible to simultaneously collect 16 million channels of digital data.

  • Optical bench needs no alignment: The C6 optical bench is compact and employs components that have evolved to be smaller, more robust, and less expensive.

  • Fluidics control: The C6 uses a unique low-pressure pumping system to drives fluidics as opposed to more expensive pressurized systems.

  • Maintenance made simple:  The C6 automatically runs Decontamination fluids through the fluidics lines and then replaces it with sheath upon instrument shutdown.

  • Works with current protocols and reagents: Accuri developed the C6 to use the same reagents you currently use on the market-leading cytometers.

  • FCS 3.0 compatible file format  

  • Compact size

  • Low power usage: The C6 Flow Cytometer operates on a low power, universal power supply (100-240V, 50-60Hz).

Additional Specifications

  • Excitation: 488nm (rated at 20,000hr life) 640nm (rated at 20,000hr life)  
  • Laser profile: 15 x 75 microns 
  • Scatter detection: Forward (0 degrees, +/-15) Side (90 degrees, +/-15)  
  • Emission detection:4 colors, user swappable optical filters  
  • Standard set installed: FL1 530 / 30nm (FITC/GFP)  
    FL2 585 / 40nm (PE/PI)  
    FL3 >670nm (PE-Cy5, PE-Cy5.5, PerCP-Cy5.5,PE-Cy5, PE-Cy7)  
    FL4 675 / 25nm (APC)  
  • Optical alignment: Fixed alignment, no maintenance required
  • Flow cell: 200 micron ID quartz  
  • Minimum particle size: 1 µm  
  • Minimum sample size: 30 µl  
  • Nominal flow rate - variable:10 to 100 µl/min ; CFlow Plus only  
  • Fixed Flow Rates & Core Sizes:  
    Slow - (Flow = 14uL/min ; Core = 10 um)  
    Medium - (Flow = 35uL/mim ; Core = 16 um)  
    Fast - (Flow = 66 uL/min ; Core = 22 um)  
  • Recommended Sheath Fluid: 0.2 um filtered DI water  
  • Core diameter: User selectable from 5 to 40 microns, CFlow Plus Only  
  • Florescence sensitivity: <750 MESF FITC  
  • Scatter resolution: Resolves human peripheral blood erythrocytes, lymphocytes, monocytes & granulocytes  
  • Fluorescence linearity:]2+/-0.05% for chick erythrocyte nuclei (CEN)  
  • Fluorescence precision:<3% CV for CEN  
  • Speed: 10,000 events/second maximum  
  • Absolute counts: Yes; CFlow Plus only  
  • Warm-up time:Less than 5 minutes  
  • Power: 70 Watts maximum, 100-240 VAC, 50/60 Hz  
  • Heat output: 240 Btu/hour maximum output  
  • Operating ranges: 15-30°C; <80% relative humidity  
  • Instrument size:11"H x 14.3"W x 16.5"D (27.9 x 36.3 x 41.9 cm)Foot print with fluid tanks 17.3"W x 16.3"D (43.9 x 41.4 cm)  
  • Weight: 30 lbs (13.6 kg)  
  • Fluid tank capacity: 1L Sheath Fluid, 1L Waste250mL Cleaner, 250mL Decontamination  
  • Signal processing:24-bit A/D conversion  
  • Computer: 17" (14.5 cm) flat-panel monitor2GB memory
  • Computer interface: USB 2.0

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