BD - FACSCalibur
by BD

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Flow cytometry

The BD FACSCalibur, a fully integrated, multiparameter system, offers the performance and sensitivity needed to ensure the objective and reproducible results vital to laboratories.

Flow cytometry has evolved rapidly to incorporate the use of multiple fluorochromes to effectively identify and isolate, with optional sorting module, ** subset populations from a single sample. This maximizes the information gained from limited amounts of sample and makes assays more cost effective.

Its optical design, sample handling options, and sorting capabilities make the BD FACSCalibur™ system a valued tool throughout the world for routine cell analysis, assay development, verification, and isolation of cellular populations of interest.

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7 years ago
Disconnected Tubing

Several times now, when I open the fluidics drawer, the tubing connected to the filter with the pinch roller attached, becomes disconnected from the filter and sheath fluid fills the drawer. Each that this has happened I reconnect the tubing and push it down as far as it will go, however, it comes off again a few weeks later. Has anyone else experienced this problem? If so, do you know what is causing the tubing to become disconnected and how can I prevent it?



9 years ago
room temperature

how does high room temperatures affect the results produced by bd facs calibur.



12 years ago
FACS calibur sensitivity
upto what concentration of antigen(in terms of microgram/ picogram) can be detected the factors which effects the senstivity.Reply


  • The BD FACSCalibur was the first system to provide standard multicolor analysis capabilities by incorporating an alignment free optical design, interbeam compensation, and dual-laser technology. Dual laser design for multicolor analysis. A dual-laser design provides the flexibility and sensitivity needed for multicolor analysis on the BD FACSCalibur system. Two lasers, an air-cooled argon laser and a red diode laser, are spatially separated for high sensitivity, minimal need for compensation, and flexibility in fluorochrome selection. BD High Throughput Sampler (HTS) option
  • The BD™ High Throughput Sampler (HTS) option provides flexible, easy-to-use automation. For those who prefer to work with multiwell plates, BD Biosciences offers the first walkaway sample introduction device for 96- and 384-well plates. The HTS option allows users to rapidly acquire samples with the BD FACSCalibur™ flow cytometer in a microtiter plate. As a modular option for new or existing BD FACSCalibur flow cytometers, the HTS option is designed to speed through a variety of microtiter plates in less than 15 minutes* and support a wide range of applications including the BD™ Cytometric Bead Array (CBA) assay. The HTS option supports 96 U-, V-, and flat-bottom plates as well as 384-well microtiter plates. BD™ Plate Manager software allows you to create customized protocols with user-defined mixing, wash, and analysis parameters that can be reused. BD FACS Loader Option 
  • The BD FACS™ Loader option available for the BD FACSCalibur system provides walkaway automation for routine applications. Featuring a removable 40-tube carousel, on-board mixing, and BD™ Worklist Manager software, the BD FACS Loader option offers superior flexibility to efficiently address the workflow demands of the busy laboratory. Unique easy-to-use sorting technology (optional)
  • The BD FACSCalibur cell sorting option allows users to identify and isolate a population of interest. Sorting on the BD FACSCalibur occurs in a completely enclosed, aerosol-free environment for enhanced safety which is especially important when processing biohazardous samples. The sorting process is designed to be straightforward. After the population of interest is gated, simply click Acquire to begin the sort. After the sample is acquired and cells pass through the laser, a unique catcher tube mechanism moves in and out of the sample core stream at a rate of roughly 300 times per second to capture designated cells and direct them to a collection tube or to an optional cell concentrator module for further processing. 
  • The BD FACSCalibur™ system is appreciated for its user-friendly design, high sample throughput, improved workflow management through automation, and simple yet sophisticated software applications that enhance routine analyses. While performing any of a wide range of supported applications, the BD FACSCalibur system provides the tools to obtain results quickly, easily, and accurately. The ease of use of the BD FACSCalibur system is enhanced by the BD FACStation™ data management system, which has become one of the most popular, user-friendly data management systems in flow cytometry. 
  • The BD FACStation system automates many software functions and delivers high-performance acquisition and analysis tools for creating plots, gating, statistical analysis, and reporting. The BD FACStation workstation takes advantage of the intuitive Mac® environment by using simple pull-down menus and icons. It performs all the computing tasks required for fast, accurate results, including instrument setup, data collection, analysis, and management. A comprehensive quality assurance system
  • BD Biosciences offers a complete family of products designed to ensure the highest quality clinical results from the BD FACSCalibur system. BD FACSComp™ software, in conjunction with BD Calibrite™ beads, provides a comprehensive system to set up the instrument, assess sensitivity, and set compensation for immunofluorescence applications. For clinical applications, BD Biosciences offers a wide variety of cellular controls to verify performance for immunophenotyping, reticulocyte analysis, stem cell enumeration, and rWBC applications.

Additional Specifications

  • Signal Processing
  • Workstation resolution:1,024 channels on all parameters. 
  • Dynamic range: Logarithmic amplifiers for SSC, FL1, FL2, FL3, and FL4 (with FL4 option), providing four log decade range. 
  • Fluorescence compensation network
  • Width and area measurements for discriminating doublets; available for all fluorescence parameters. 
  • Rack support:Up to 16 racks per BD FACS Loader. 
  • Data entry: Sample information, reagent panels, and rack information can be defined for up to 640 tubes (40 tubes x 16 racks) at a time. 
  • Automated control through BD™ Worklist Manager software. 
  • Barcode scanner (optional)
  • Automates data entry for Codabar, Code 39, Interleaved 2 of 5, Code 2 of 5, and Code 128. 
  • Three modes (all aerosol free): single cell, exclusion, and recovery. 
  • Recovery
  • Sterile sorting
  • System design allows for aerosol-free sterile sorting. 
  • Mac Pro computer Processor 2.8-GHz Quad-Core Intel® Xeon® processor 
  • Memory: 2 GB RAM 
  • 4 MB per processor 
  • Sensor module (W x D x H): 91.4 x 61.5 x 67.3 cm (36 x 24.2 x 26.5 in); 
  • Height with cover option: 124.5 cm (49 in) 
  • Computer: (W x D x H): 48 x 41 x 58 cm (19 x 16 x 23 in) 
  • Printer (W x D x H): 48 x 41 x 58 cm (19 x 16 x 23 in) 

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