Slide Stainer/Prep Forums

3 Replies

A client has a Leica 5010 which frequently reinitializes, very early into a program, and then usually errors with "Head stalled" or "Make sure that head is free of obstructions," which usually occurs at the extreme left or right end of X travel (but before it hits a physical stop). I have lubricated the guide rods and linear bearings - they are moving pretty freely. I have tried flexing the loom, while it was in motion, to see if there is any place where flexing causes an error, but have not found one. There are a couple of power resistors on the Head pcb - I resoldered those, just in case. I have cleaned and reseated various connectors. Has anyone run into this? Is it likely to be either the motor or the main control pcb?

RE: Leica Biosystems - ST5010 Autostainer XL
Updated  Aug 18 2025
1 Reply

How to start the varistain 24 ?

Equipment: Thermo Scientific - Varistain V24-4

RE: Thermo Scientific - Varistain V24-4
1 Reply

HELLO, DOES ANYONE KNOW WHY IT DOESN'T SAVE WORK PROGRAMS THEY GET DELETED FROM MEMORY?

THANK YOU.

RE: Leica Biosystems - ST5010 Autostainer XL
0 Replies

In case there is anyone from MedWrench monitoring/moderating these forums...
These are actually two separate instruments. The Sakura Prisma (model 6130 or 6131) or Prisma Plus (6170 or 6171) is the stainer. It can be purchased or used in stand-alone configuration, or it can be married with either a glass coverslipper (GLAS 6500), or with a film coverslipper (Film 4740); each of those coverslippers can be purchased or used in a stand-alone configuration. 

RE: Sakura - Tissue-Tek Prisma/Film
7 Replies

Hi Team

I have been trying desperately to problem solve a daily/weekly issue with this coverslipper - to the point where the actual tech who comes to the lab who is contracted to fix the issues, has liaised with me about the issue and is telling me that I am doing the right thing with maintenance, and solving the issue (usually I can clear it, but with one CS2 it is just constantly jamming.

So what happens is it comes up with the motor error, and I see that no slides have actually been coverslipped - and back toward where the wheel 7 is connected to the little black wheel, it seems to build up glue? I can literally dry touch it with my gloves and it sticks to it at the black wheel. So I’ve been cleaning the wheels with xylene as it’s shut off to allow wheel separation and movement, but the issue persists. And that issue is after that movement of the film through this particular stage, it is somehow doubling over, instead of ejecting the film onto each slide. I’ve tested it with say, 5 blank slides, after the clean, and the build up of film is horrendous. I’m not sure how I can post a picture of this to accurately show what is going on, but it is currently doing my head in, and it’s almost like the tech doesn’t know what he’s doing either, so it’s been left to me, the only sci who knows how to fiddle around with it.

I have to twist my forceps around at the end before the blade to actually remove these doubled/tripled/9000 film build ups that occur, changing the entire tape does nothing, so that leads me to believe that there’s something going on underneath the stage that the film slides through as it gets to the point of glue dispensing (or am I wrong with where the glue gets dispensed here, I am not formally educated on the machine, this is all me trying desperately to problem solve the machine time and time again without much help of the company contacted tech). To be clear, the stage I am talking about is metal with black (plastic?) screwed down, and as you press the film button on the machine, sometimes I hear it clanging and pushing the film too fast (is that an issue to be addressed?) it usually stops when I cease and wait, then slowly press the button again. It comes out fine as I do this, but I do notice this “streaking” on the film that I touch, and again, it is often sticky. So that also leads me to believe that this is somehow a glue dispensing issue, or I actually need to unscrew that stage and see what’s going on underneath there. 

I hope I have explained myself thoroughly enough as I have to go try and take this machine back out of order tomorrow morning and get it fixed within 30 minutes which is my current permitted timeframe to perform weekly maintenance on the machine. 

The other issue I guess is undertrained staff trying to fix it themselves as I am too busy doing my routine work and boss is more concerned that I keep my kpis than ensuring the machine health of a very expensive piece of equipment - but we have talked about holding some sort of meeting about how to properly handle the machine.

Any replies, advice, recommendations, help would be absolutely, and desperately appreciated at this stage as it is ruining my life, haha. 

This is my first post, unsure of what is allowed regarding to pictures or if possible to upload, so if that also could be clarified that would be great. Just wish to show how I mean by the film doubling over before it gets to the blade, and the result of film build up that I have to clear out of that. 

Thank you to any and all, wish me luck, I need it :(

Kind Regards,

Dan

RE: Sakura - Tissue-Tek Prisma/Film
Updated  Jun 4 2025
1 Reply

I need a copy of the user manual for the previ color gram v2.

Equipment: previ color gram v2

RE: Biomerieux - Previ Color Gram
Updated  Apr 26 2025
2 Replies

Hello all . Does anyone know thé référence of thé lcd screen because i have thé screen broken and i need to search a similar screen and i need thé référence . Thank u 

RE: Sakura - Tissue-Tek Prisma/Film
5 Replies

Was looking for a place to purchase the gear assemblies or if someone knows if Siemens changed the part numbers when they purchased them from Bayer.

RE: Siemens - Hema-Tek 2000
Added  Dec 4 2024
0 Replies

Hello!
I have an issue with this machine and I hope someone might be able to help me.
When I turn on the machine, the display gives a very short flash of light but that's it, no further action. Does anyone know what this is? The manual doesn't cover this and I've checked all the logical options (fuses, properly plugged in etc)

RE: Leica Biosystems - ST5010 Autostainer XL
Updated  Aug 21 2024
1 Reply

Does anybody know what could cause an E008 error? 

RE: Biomerieux - Previ Color Gram

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