Cryostat Forums

Updated  Apr 1 2024
2 Replies

We seem to need a recharge. Taking the front plate off I can see it takes 404a but I don't know the volume it takes. Anyone have an idea?

RE: Microm - HM 505 E
2 Replies

Sakura Cryo3 Flex cryostat.

I am looking for manuals (service and/or user), PM checklist, or information describing differences in operation, service, or parts between the Flex version and the original version. 

I have been asked to PM a Cryo 3 Flex. I can see a few differences, but I'm not sure what else there might be, and what I would want to do differently in a PM.

RE: Sakura - Tissue-Tek Cryo3
Updated  Feb 5 2024
6 Replies

HI.

I have a problem on the microtome. When you try to make cuts, the microtome sometimes cut a thick slice and then doesn't cut at all after 4 or 5 turns, and then again a thick cut. 

I thought the problem was in the screw ot the nut that moves the arm forward, but those parts seems to be ok.

Maybe somebody had this problem in the past an knows how to solve it.


RE: Thermo Fisher Scientific - Cryotome E
Updated  Jan 12 2024
1 Reply

I noted that a fairly new Leica 1520 comes with 315 grams of R452A refrigerant. Does anyone know if this will be the norm - will all of the cryostat manufacturers be switching to this refrigerant, instead of R404A (which is about to be phased out)?
I did note that it does still use a Danfoss SC15G compressor (which now has a sticker on it saying "R452A"). And, unfortunately, it also still has the double e-valve.

RE: Leica Biosystems - CM1520
1 Reply

leica cm3050S --motor stalled was completely locked up,I replaced motor but still not functioning, is there a motor drive board or fuse besides the 4 under the left panel that i can check 

RE: Leica Biosystems - CM3050 S
Updated  Jul 11 2023
4 Replies

I need a temperature probe for Microm HM520 or HM525  ( part number 330320). The probe is not available from the thermo fisher scientific. Does anybody know the datasheet for this probe, so I can find a compatible one or have it remanufactured?

RE: Richard-Allan Scientific - Microm HM525 NX
Updated  Jun 23 2023
1 Reply

Has anyone experienced on the NX50 the service programming going blank?  We were told by the manufacturer that the firmware needs to be re-entered with a thumb drive.  Does anyone have this thumb drive.  Manufacturer is not being forthcoming with drive details.  


Equipment: Thermo Fisher Scientific - NX50 Cryostat

RE: Thermo Fisher Scientific - NX50 Cryostat
3 Replies

CABINET TEMPERATURE NOT GETTING -23, ROOM TEMPERATURE IS ALMOST 26deg C

Equipment: lEICA CM1850

RE: Leica Biosystems - CM1850
Updated  Oct 24 2022
1 Reply

my machine does not turn off when it reaches the programmed temperature

Equipment: Microm - HM 505 E

RE: Microm - HM 505 E
Updated  Sep 8 2022
2 Replies

I have the Cryo3 Plus service manual but this machine is different. is is showing an Err:07 configuration error, I need the service manual to find out what it is.

Equipment: Sakura Tissue-tek Cryo3 FLEX

RE: Sakura - Tissue-Tek Cryo3

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