Absorbance out of limits
Equipment: BioSystems - BTS 330
Replace lamp , If absorbance out of limits on all filters , if not , absorbance out of limit for one particular filter change the filter , Most probably the this problem will arise for 340nm (trhe filter has become opaque).
2. Clean the Flow cell , throughly, The absorbance of Flow cell with distilled water , shall not exceed 0.3 in 340 nm